Each mouse was subcutaneously injected with 200 µl cell suspension of 5 × 10 6 A375 vector or OE-PKNOX1-1 cells into the armpit of the right forelimb.

Biotin contributes to the maintenance of normal hair, skin and mucous membranes, and takes part in protein, carbohydrate and fat metabolism. Finance is provided by PayPal Credit (a trading name of PayPal UK Ltd, Whittaker House, Whittaker Avenue, Richmond-Upon-Thames, Surrey, United Kingdom, TW9 1EH). After incubation in primary antibodies at 4°C overnight, the membranes were incubated with HRP-conjugated anti-rabbit secondary antibody (1:5000; Cell Signaling Technology, USA) at room temperature for 1 h.

For ethynyldeoxyuridine (EdU) assay, the cells were seeded into 96-well plates at 5 × 10 5 cells per well, and incubated with 10 µM EdU at 37°C for 2 h, then fixed and permeated.

The results above were from at least three independent repeats and were obtained through analyses performed using the one- and two-way ANOVA. You can change your choices at any time by visiting Cookie preferences, as described in the Cookie notice.

This animal experiment program obtained the consent of the Animal Care and Use Committee of our hospital (approval number: 2021-DW-003). And if you’re looking for a signature scent, Costco has a large selection of men’s colognes and women’s perfumes from popular brands.

The sequences of the lnc-PKNOX1-1 probes are shown in Supplementary Table 1, available at Carcinogenesis Online.

The software programs used for statistical analyses were Microsoft Office Excel, IBM SPSS Statistics 26, GraphPad Prism6 and ImageJ. LncRNAs in nucleus exert their functions mainly through regulating chromosome structure and function, and cis-/ trans-regulating gene transcription. Rescue assays proved that IL-8 overexpression partially reversed the tumor-inhibitory function of lnc-PKNOX1-1 in melanoma cells, implying that lnc-PKNOX1-1 could suppress melanoma development by regulating IL-8. Effect of lnc-PKNOX1-1 on tumor growth of melanoma by in vivo assay, and identification of DEGs by microarray analysis, enrichment analysis, western blot and ELISA.