200X Phone Microscope Lens with LED Light Portable Digital Microscope for Kids Handheld Microscope Dermatoscope Skin Diagnosis Hair Analyzer Compatible with iPhone and Android Mobile Phone(Black)

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Wang, P. & Menon, R. Computational spectrometer based on a broadband diffractive optic. Opt. Express 22, 14575 (2014). Bianco, I. H., Kampff, A. R. & Engert, F. Prey capture behavior evoked by simple visual stimuli in larval zebrafish. Front. Syst. Neurosci. 5, 101 (2011). Anger, P., Bharadwaj, P. & Novotny, L. Enhancement and quenching of single-molecule fluorescence. Phys. Rev. Lett. 96, 113002 (2006). Yekti, A. P. A., Hsu, H.-J. & Wang, W.-D. The effect of paclobutrazol on the development of zebrafish (Danio rerio) embryos. Zebrafish 11, 1–9 (2014).

To test the system’s adaptability on a different fluorescence spectrum, Rhodamine-123 ( Excitation 507 nm, Emission 529 nm) was also used to measure beta-cell mitochondrial potentials changes. As shown in Figure5F, fluorescence intensity dropped to 89.2% (88.5% ± 2.8) when stimulated with 14 mM glucose at 5min. The result captured by our system correctly reflected the mitochondrial potential hyperpolarization post glucose stimulation, which suggested that the smartphone system was able to monitor fluorescence signals of different wavelengths. Fluorescence imaging of islets labeled with GEPFIs Douglas, S. M. et al. Rapid prototyping of 3D DNA-origami shapes with caDNAno. Nucleic Acids Res. 37, 5001–5006 (2009).

Two smartphone adapter versions

Rabha, D., Sarmah, A. & Nath, P. Design of a 3D printed smartphone microscopic system with enhanced imaging ability for biomedical applications. J. Microsc. 276, 13–20 (2019).

Plöschner, M., Tyc, T. & Čižmár, T. Seeing through chaos in multimode fibres. Nat. Photonics 9, 529–535 (2015). Département de Physique - Photonic Nanosystems, Université de Fribourg - Faculté des Sciences et Médicine, Fribourg, Switzerland Kurokawa, U., Choi, B. I. L. & Chang, C.-C. Filter-based miniature spectrometers: Spectrum reconstruction using adaptive regularization. IEEE Sens. J. 11, 1556–1563 (2011). Fereidouni, F., Mitra, A. D., Demos, S. & Levenson, R. Microscopy with UV surface excitation (MUSE) for slide-free histology and pathology imaging. in Optical Biopsy XIII: Toward Real-Time Spectroscopic Imaging and Diagnosis vol. 9318 93180F (International Society for Optics and Photonics, 2015). Taylor, A. B. & Zijlstra, P. Single-molecule plasmon sensing: current status and future prospects. ACS Sens. 2, 1103–1122 (2017).Bauerle, C. et al. Vision and Change in Undergraduate Biology Education: A Call to Action. American Association for the Advancement of Science https://visionandchange.orgfinalreport (2021). Di Febo, R., Casas, L. & Antonini, A. A smartphone-based petrographic microscope. Microsc. Res. Tech. 84, 1414–1421 (2021). The total distance between the ocular lens and the objective lens was calculated as follows: u 1 + v 0=80 mm, which provided enough space to fit a dichroic cube (60mm x 60mm x 60mm). Under such setup, the size of the field of view was measured to be roughly 0.8mm x 0.8mm.